Faculty

Rotem Karni, PhD

Associate Professor of Genetics

Department: Genetics

Graduate Group Affiliations

Cell and Molecular Biology

Contact information

One Ucity square, floor 4, room 4018
Philadelphia, 19104

Education:
BsC
The Hebrew University of Jerusalem, 1997.
Ph.D. (Biological Chemistry)
The Hebrew University of Jerusalem, Israel., 2002.

Links
Lab webstite

Permanent link

> Perelman School of Medicine > Faculty > Details

Selected Publications

Shivang Khandelwal, Ela Elyada, Reut Japha, Manar Abu Diab, Anurag Prabhu, Zahava Siegfried and Rotem Karni.: Molecular characterization of the heterozygous loss of function mutations in the X-linked PCDH19 gene causing PCDH19-Cluster Epilepsy. bioRxiv 2026 Notes: 2026.03.16 (uploaded).

Prabhu, A.V., Azar-Koussa, C., Siegfried, Z. and Karni R. : Spliced to kill: RNA mis-splicing derived cancer neoantigens. Trends Cancer. 11: 611-613, 2025.

Shay Tzaban, Priyanga Appasamy, Elad Zisman, Shiri Klein, Reyut Lewis, Houlin Yu, Akanksha Khorgade, Marc A. Schwartz, Moshe Sade-Feldman, Thomas Eisenhaure, Oren Parnas, Aron Popovtzer, Cyrille Cohen, Eric Shifrut, Aziz M. Al’Khafaji, Rotem karni, Galit Eisenberg, Nir Hacohen, Michal Lotem.: RNA splicing dynamics in CD8 T cells uncovers isoforms that impact T cell-mediated cancer immunotherapy. bioRxiv 2025 Notes: 2025.09.07.674706; doi: https://doi.org/10.1101/2025.09.07.674706.

Yehezkel Sztainberg, Maya David Teitelbaum, Ilana Buchumenski, Hagit Porath, Dror Sharon, Eyal Banin, Rotem Karni, Erez Y. Levanon, Ariel Feiglin.: The prevalent NR2E3 c.932G>A mutation induces aberrant splicing that can be rescued using splice-shifting antisense oligonucleotides. bioRxiv 2024 Notes: 2024.05.01.592034; doi: https://doi.org/10.1101/2024.05.01.592034.

Rosenberg-Mogilevsky, A., Siegfried, Z. and Karni R.: Generation of tumor neoantigens by RNA splicing perturbation. Trends Cancer. 11:12-24 11: 12-24, 2024.

Yu KM, Trimble DJ, Conner CR, Sheinberg DL, Low GMI, Saluja K, Schmitt KM, Karni RJ.: Oligometastatic Parathyroid Carcinoma Presenting as Neck Pain: A Case Report. Ear Nose Throat J 102(19): 635-639, Oct 2023.

Amar-Schwartz A, Cohen Y, Elhaj A, Ben-Hur V, Siegfried Z, Karni R, Dor T.: Inhibition of nonsense-mediated mRNA decay may improve stop codon read-through therapy for Duchenne muscular dystrophy. Hum Mol Genet 32(15): 2455-2463, Jul 2023.

Jbara A, Lin KT, Stossel C, Siegfried Z, Shqerat H, Amar-Schwartz A, Elyada E, Mogilevsky M, Raitses-Gurevich M, Johnson JL, Yaron TM, Ovadia O, Jang GH, Danan-Gotthold M, Cantley LC, Levanon EY, Gallinger S, Krainer AR, Golan T, Karni R.: RBFOX2 modulates a metastatic signature of alternative splicing in pancreatic cancer. Nature 617(7959): 147-153, May 2023.

Bashari A, Siegfried Z, Karni R.: Targeting splicing factors for cancer therapy. RNA 29(4): 506-515, Apr 2023.

Bashari A, Siegfried Z, Karni R.: Targeting splicing factors for cancer therapy. RNA 29(4): 506-515, Apr 2023.